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Plates were read at 405 nm and titers were defined as the log 3 serum dilution two-fold above background

Definitive diagnosis can be confirmed with skin biopsy that typically shows any of the following: Evidence of neutrophilic infiltration within and around the vessel wall with the signs of clear dust or leukocytoclasia (disintegration of neutrophil nuclei into fragments) Fibrinoid necrosis or fibrin deposition within and around the vessel wall Signs of damage to the vessel wall and surrounding tissue such as damaged endothelial cells or extravasated red blood cells

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